Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients : the European Early Lung Cancer Project

Van Den Berg, Remco M., Brokx, Hes, Vesin, Aurelien, Field, John K., Brambilla, Christian, Meijer, Chris J.L.M., Sutedja, G. Thomas, Heideman, Danielle A.M., Postmus, Pieter E., Smit, Egbert F., Snijders, Peter J.F., Martinet, Yves, Thunnissen, Frederick B., Sozzi, Gabriella, Risch, Angela, Becker, Heinrich D., Elborn, J. Stuart, Montuenga, Luis M., O'Byrne, Ken J., Harrison, David J., & Niklinski, Jacek (2010) Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients : the European Early Lung Cancer Project. International Journal of Oncology, 37(2), pp. 455-461.

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Lung cancer is the most important cause of cancer-related mortality. Resectability and eligibility for treatment with adjuvant chemotherapy is determined by staging according to the TNM classification. Other determinants of tumour behaviour that predict disease outcome, such as molecular markers, may improve decision-making. Activation of the gene encoding human telomerase reverse transcriptase (hTERT) is implicated in the pathogenesis of lung cancer, and consequently detection of hTERT mRNA might have prognostic value for patients with early stage lung cancer. A cohort of patients who underwent a complete resection for early stage lung cancer was recruited as part of the European Early Lung Cancer (EUELC) project. In 166 patients expression of hTERT mRNA was determined in tumour tissue by quantitative real-time RT-PCR and related to that of a house-keeping gene (PBGD). Of a subgroup of 130 patients tumour-distant normal tissue was additionally available for hTERT mRNA analysis. The correlation between hTERT levels of surgical samples and disease-free survival was determined using a Fine and Gray hazard model. Although hTERT mRNA positivity in tumour tissue was significantly associated with clinical stage (Fisher's exact test p=0.016), neither hTERT mRNA detectability nor hTERT mRNA levels in tumour tissue were associated with clinical outcome. Conversely, hTERT positivity in adjacent normal samples was associated with progressive disease, 28% of patients with progressive disease versus 7.5% of disease-free patients had detectable hTERT mRNA in normal tissue [adjusted HR: 3.60 (1.64-7.94), p=0.0015]. hTERT mRNA level in tumour tissue has no prognostic value for patients with early stage lung cancer. However, detection of hTERT mRNA expression in tumour-distant normal lung tissue may indicate an increased risk of progressive disease.

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5 citations in Web of Science®
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ID Code: 65780
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Disease-free survival, hTERT, Non-small cell lung cancer, Prognosis, RT-PCR, messenger RNA, telomerase reverse transcriptase, adult, aged, article, cancer surgery, cancer survival, controlled study, correlation analysis, disease free survival, female, human, lung cancer, major clinical study, male, priority journal, protein expression, reverse transcription polymerase chain reaction
DOI: 10.3892/ijo_00000694
ISSN: 1019-6439
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2010 Spandidos Publications
Deposited On: 08 Jan 2014 02:14
Last Modified: 13 Mar 2014 03:45

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