Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA

Waterhouse, P. M., Graham, M. W., & Wang, M. B. (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proceedings of the National Academy of Sciences of the United States of America, 95(23), pp. 13959-13964.

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Abstract

Many examples of extreme virus resistance and posttranscriptional gene silencing of endogenous or reporter genes have been described in transgenic plants containing sense or antisense transgenes. In these cases of either cosuppression or antisense suppression, there appears to be induction of a surveillance system within the plant that specifically degrades both the transgene and target RNAs. We show that transforming plants with virus or reporter gene constructs that produce RNAs capable of duplex formation confer virus immunity or gene silencing on the plants. This was accomplished by using transcripts from one sense gene and one antisense gene colocated in the plant genome, a single transcript that has self-complementarity, or sense and antisense transcripts from genes brought together by crossing. A model is presented that is consistent with our data and those of other workers, describing the processes of induction and execution of posttranscriptional gene silencing.

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ID Code: 65796
Item Type: Journal Article
Refereed: Yes
Additional Information: Open Access copy of this article is available via PubMed Central (see Link below)
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Keywords: complementary dna, complementary rna, transcription factor, article, gene expression, genome, plant, plasmid, priority journal, reporter gene, silencer gene, transgenic plant, virus immunity, virus resistance
DOI: 10.1073/pnas.95.23.13959
ISSN: 0027-8424
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: The National Academy of Sciences
Deposited On: 08 Jan 2014 01:41
Last Modified: 08 Jan 2014 01:43

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