Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

Loss-Morais, G., Waterhouse, P. M., & Margis, R. (2013) Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets. Biology Direct, 8(1).

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Abstract

tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants. © 2013 Loss-Morais et al.; licensee BioMed Central Ltd.

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ID Code: 65812
Item Type: Journal Article
Refereed: Yes
Keywords: Argonaute and Arabidopsis, Small RNA, TRFs, TRNA-derived RNA fragments, TRNAs, Arabidopsis, Arabidopsis thaliana, argonaute protein, microRNA, transfer RNA, article, biology, gene library, genetics, high throughput sequencing, immunology, immunoprecipitation, metabolism, microbiology, physiology, Pseudomonas syringae, Argonaute Proteins, Computational Biology, High-Throughput Nucleotide Sequencing, MicroRNAs, RNA, Transfer
DOI: 10.1186/1745-6150-8-6
ISSN: 1745-6150
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: The authors
Deposited On: 08 Jan 2014 03:31
Last Modified: 09 Jan 2014 03:16

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