DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana

Eamens, A. L., Kim, K. W., & Waterhouse, P. M. (2012) DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana. Plant Signaling and Behavior, 7(10).

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Abstract

DOUBLE-STRANDED RNA BIN DIN G (DRB) proteins have been functionally characterized in viruses, prokaryotes and eukaryotes and are involved in all aspects of RNA biology. Arabidopsis thaliana (Arabidopsis) encodes five closely related DRB proteins, DRB1 to DRB5. DRB1 and DRB4 are required by DICER-LIKE (DCL) proteins DCL1 and DCL4 to accurately and efficiently process structurally distinct double-stranded RNA (dsRNA) precursor substrates in the microRNA (miRNA) and trans-acting small-interfering RNA (tasiRNA) biogenesis pathways respectively. We recently reported that DRB2 is also involved in the biogenesis of specific miRNA subsets. Furthermore, the severity of the developmental phenotype displayed by the drb235 triple mutant plant, compared with those expressed by either drb2, drb3 and drb5 single mutants, or double mutant combinations thereof, indicates that DRB3 and DRB5 function in the same non-canonical miRNA pathway as DRB2. Through the use of our artificial miRNA (amiRNA) plant expression vector, pBlueGreen 2,3 we demonstrate here that unlike DRB2, DRB3 and DRB5 are not involved in the dsRNA processing stages of the miRNA biogenesis pathway, but are required to mediate RNA silencing of target genes of DRB2-associated miRNA s. © 2012 Landes Bioscience.

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ID Code: 65813
Item Type: Journal Article
Refereed: Yes
Additional Information: Open access copy available via BioMed Central (BMC)
Additional URLs:
Keywords: Arabidopsis, Artificial miRNA, Double-stranded RNA, Double-stranded RNA binding protein, MicroRNA, Noncanonical, RNA silencing, PMCID: PMC3493401
DOI: 10.4161/psb.21518
ISSN: 1559-2316
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Landes Bioscience
Deposited On: 08 Jan 2014 03:26
Last Modified: 03 Apr 2014 04:19

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