RNA interference-inducing hairpin RNAs in plants act through the viral defence pathway

Fusaro, A. F., Matthew, L., Smith, N. A., Curtin, S. J., Dedic-Hagan, J., Ellacott, G. A., Watson, J. M., Wang, M. B., Brosnan, C., Carroll, B. J., & Waterhouse, P. M. (2006) RNA interference-inducing hairpin RNAs in plants act through the viral defence pathway. EMBO Reports, 7(11), pp. 1168-1175.

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RNA interference (RNAi) is widely used to silence genes in plants and animals. It operates through the degradation of target mRNA by endonuclease complexes guided by approximately 21 nucleotide (nt) short interfering RNAs (siRNAs). A similar process regulates the expression of some developmental genes through approximately 21 nt microRNAs. Plants have four types of Dicer-like (DCL) enzyme, each producing small RNAs with different functions. Here, we show that DCL2, DCL3 and DCL4 in Arabidopsis process both replicating viral RNAs and RNAi-inducing hairpin RNAs (hpRNAs) into 22-, 24- and 21 nt siRNAs, respectively, and that loss of both DCL2 and DCL4 activities is required to negate RNAi and to release the plant's repression of viral replication. We also show that hpRNAs, similar to viral infection, can engender long-distance silencing signals and that hpRNA-induced silencing is suppressed by the expression of a virus-derived suppressor protein. These findings indicate that hpRNA-mediated RNAi in plants operates through the viral defence pathway.

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ID Code: 65817
Item Type: Journal Article
Refereed: Yes
Additional Information: Open Access copy of this article is available via PubMed Central
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Keywords: dicer like enzyme 2, dicer like enzyme 3, dicer like enzyme 4, microRNA, plant RNA, small interfering RNA, unclassified drug, vegetable protein, virus RNA, Arabidopsis, article, enzyme activity, gene expression, gene repression, gene silencing, nonhuman, plant genetics, priority journal, real time polymerase chain reaction, RNA interference, RNA replication, viral plant disease, virus replication, Arabidopsis Proteins, Cell Cycle Proteins, Gene Expression Regulation, Plant, Models, Genetic, Plant Roots, Plant Shoots, Plant Viruses, Ribonuclease III, Ribonucleases, RNA, Plant, Animalia, PMCID: PMC1679793, OA via PMC
DOI: 10.1038/sj.embor.7400837
ISSN: 1469-221X
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: European Molecular Biology Organization
Deposited On: 08 Jan 2014 03:02
Last Modified: 22 Jan 2015 05:48

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