Constructs and methods for hairpin RNA-mediated gene silencing in plants

Helliwell, C. A. & Waterhouse, P. M. (2005) Constructs and methods for hairpin RNA-mediated gene silencing in plants. Methods in enzymology, 392, pp. 24-35.

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Double-stranded RNA (dsRNA) induces an endogenous sequence-specific RNA degradation mechanism in most eukaryotic cells. The mechanism can be harnessed to silence genes in plants by expressing self-complementary single-stranded (hairpin) RNA in which the duplexed region has the same sequence as part of the target gene's mRNA. We describe a number of plasmid vectors for generating hairpin RNAs, including those designed for high-throughput cloning, and provide protocols for their use.

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ID Code: 65848
Item Type: Journal Article
Refereed: Yes
Keywords: double stranded RNA, article, controlled study, gene construct, gene expression, gene sequence, gene silencing, gene targeting, high throughput screening, molecular cloning, nonhuman, priority journal, RNA degradation, Base Sequence, DNA Primers, Genetic Vectors, Nucleic Acid Conformation, Plants, RNA, Plant, Eukaryota
DOI: 10.1016/S0076-6879(04)92002-2
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Elsevier
Deposited On: 09 Jan 2014 07:51
Last Modified: 29 Jan 2014 00:26

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