C. elegans RNA-dependent RNA polymerases rrf-1 and ego-1 silence Drosophila transgenes by differing mechanisms

Duan, Guowen, Saint, Robert B., Helliwell, Chris A., Behm, Carolyn A., Wang, Ming B., Waterhouse, Peter M., & Gordon, Karl H. J. (2013) C. elegans RNA-dependent RNA polymerases rrf-1 and ego-1 silence Drosophila transgenes by differing mechanisms. Cellular and Molecular Life Sciences, 70(8), pp. 1469-1481.

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Abstract

Drosophila possesses the core gene silencing machinery but, like all insects, lacks the canonical RNA-dependent RNA polymerases (RdRps) that in C. elegans either trigger or enhance two major small RNA-dependent gene silencing pathways. Introduction of two different nematode RdRps into Drosophila showed them to be functional, resulting in differing silencing activities. While RRF-1 enhanced transitive dsRNA-dependent silencing, EGO-1 triggered dsRNA-independent silencing, specifically of transgenes. The strain w; da-Gal4; UAST-ego-1, constitutively expressing ego-1, is capable of silencing transgene including dsRNA hairpin upon a single cross, which created a powerful tool for research in Drosophila. In C. elegans, EGO-1 is involved in transcriptional gene silencing (TGS) of chromosome regions that are unpaired during meiosis. There was no opportunity for meiotic interactions involving EGO-1 in Drosophila that would explain the observed transgene silencing. Transgene DNA is, however, unpaired during the pairing of chromosomes in embryonic mitosis that is an unusual characteristic of Diptera, suggesting that in Drosophila, EGO-1 triggers transcriptional silencing of unpaired DNA during embryonic mitosis. © 2012 Springer Basel.

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ID Code: 65911
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Drosophila, microRNAs (miRNAs), RNA interference (RNAi), RNA-dependent RNA polymerase (RdRp), DNA, ego 1 protein, RNA directed RNA polymerase, rrf 1 protein, unclassified drug, article, chromosome pairing, controlled study, EGFP gene, enzyme activity, gene, gene rearrangement, gene silencing, mitosis, NaChaBac gene, nonhuman, pebble gene, protein expression, RNA interference, transgene, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Drosophila Proteins, Genes, Reporter, Green Fluorescent Proteins, Guanine Nucleotide Exchange Factors, MicroRNAs, RNA Replicase, RNA, Small Interfering, Transgenes, Diptera, Hexapoda
DOI: 10.1007/s00018-012-1218-8
ISSN: 1420-682X
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Birkhaeuser Science
Deposited On: 09 Jan 2014 02:06
Last Modified: 13 Jan 2014 04:16

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