The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation

Fusaro, Adriana F., Correa, Regis L., Nakasugi, Kenlee, Jackson, Craig, Kawchuk, Lawrence, Vaslin, Maite F. S., & Waterhouse, Peter M. (2012) The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation. Virology, 426(2), pp. 178-187.

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Abstract

The P0 protein of poleroviruses and P1 protein of sobemoviruses suppress the plant's RNA silencing machinery. Here we identified a silencing suppressor protein (SSP), P0PE, in the Enamovirus Pea enation mosaic virus-1 (PEMV-1) and showed that it and the P0s of poleroviruses Potato leaf roll virus and Cereal yellow dwarf virus have strong local and systemic SSP activity, while the P1 of Sobemovirus Southern bean mosaic virus supresses systemic silencing. The nuclear localized P0PE has no discernable sequence conservation with known SSPs, but proved to be a strong suppressor of local silencing and a moderate suppressor of systemic silencing. Like the P0s from poleroviruses, P0PE destabilizes AGO1 and this action is mediated by an F-box-like domain. Therefore, despite the lack of any sequence similarity, the poleroviral and enamoviral SSPs have a conserved mode of action upon the RNA silencing machinery. © 2012 Elsevier Inc.

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ID Code: 65912
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Enamovirus, Luteoviridae, P0, Polerovirus, Silencing suppression, Sobemovirus, argonaute 1 protein, F box protein, protein po, unclassified drug, virus protein, amino acid sequence, article, cell nucleus, Cereal yellow dwarf virus, controlled study, gene silencing, nonhuman, nucleotide sequence, Pea enation mosaic virus 1, priority journal, protein degradation, protein determination, protein domain, protein function, protein localization, protein RNA binding, protein stability, sequence homology, southern bean mosaic virus, Arabidopsis, Arabidopsis Proteins, Argonaute Proteins, Molecular Sequence Data, Plant Diseases, Repressor Proteins, RNA Interference, Sequence Alignment, Viral Proteins, Pea enation mosaic virus-1, Potato leafroll virus
DOI: 10.1016/j.virol.2012.01.026
ISSN: 0042-6822
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2012 Academic Press
Deposited On: 09 Jan 2014 02:14
Last Modified: 03 Apr 2014 04:59

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