Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development
Duan, Guowen, Saint, Robert B., Helliwell, Chris A., Behm, Carolyn A., Waterhouse, Peter M., & Gordon, Karl H. J. (2010) Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development. Transgenic Research, 19(6), pp. 1121-1128.
Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT-PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development. © 2010 Springer Science+Business Media B.V.
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|Item Type:||Journal Article|
|Keywords:||MicroRNAs (miRNAs), RNA interference (RNAi), RNA-directed RNA polymerase (RdRp), Caenorhabditis elegans protein, EGO 1 protein, C elegans, EGO-1 protein, C elegans, helminth DNA, recombinant protein, RNA directed RNA polymerase, animal, article, Caenorhabditis elegans, Drosophila melanogaster, enzymology, female, gene, gene expression, genetics, growth, development and aging, male, metabolism, morphogenesis, nucleotide sequence, phylogeny, physiology, RNA interference, transgenic animal, Animals, Animals, Genetically Modified, Base Sequence, Caenorhabditis elegans Proteins, DNA, Helminth, Genes, Helminth, Recombinant Proteins, RNA Replicase, Hexapoda, Vertebrata|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2010 Springer Verlag|
|Deposited On:||09 Jan 2014 02:33|
|Last Modified:||05 Mar 2014 04:17|
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