Cloning and characterization of microRNAs from Brassica napus

Wang, Lei, Wang, Ming-Bo, Tu, Jin-Xing, Helliwell, Christopher A., Waterhouse, Peter M., Dennis, Elizabeth S., Fu, Ting-Dong, & Fan, Yun-Liu (2007) Cloning and characterization of microRNAs from Brassica napus. FEBS Letters, 581(20), pp. 3848-3856.

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Abstract

A library containing approximately 40,000 small RNA sequences was constructed for Brassica napus. Analysis of 3025 sequences obtained from this library resulted in the identification of 11 conserved miRNA families, which were validated by secondary structure prediction using surrounding sequences in the Brassica genome. Two 21 nt small RNA sequences reside within the arm of a pre-miRNA like stem-loop structure, making them likely candidates for novel non-conserved miRNAs in B. napus. Most of the conserved miRNAs were expressed at similar levels in a F1 hybrid B. napus line and its four double haploid progeny that showed marked variations in phenotypes, but many were differentially expressed between B. napus and Arabidopsis. The miR169 family was expressed at high levels in young leaves and stems, but was undetectable in roots and mature leaves, suggesting that miR169 expression is developmentally regulated in B. napus. © 2007 Federation of European Biochemical Societies.

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ID Code: 65951
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Brassica napus, Heterosis, MicroRNAs, miR169, Secondary structure, Small RNAs, hybrid protein, microRNA, Arabidopsis, article, gene construct, gene library, gene structure, genetic conservation, genetic variability, haploidy, molecular clock, nonhuman, plant leaf, plant root, plant stem, priority journal, progeny, protein analysis, protein expression, rapeseed, RNA sequence, Base Sequence, Cloning, Molecular, Conserved Sequence, Gene Expression Regulation, Plant, Nucleic Acid Conformation, Phylogeny, RNA, Plant, Sequence Analysis, RNA, Brassica
DOI: 10.1016/j.febslet.2007.07.010
ISSN: 0014-5793
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2007 Elsevier BV
Deposited On: 09 Jan 2014 03:41
Last Modified: 09 Jan 2014 03:44

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