A suite of novel promoters and terminators for plant biotechnology II. The pPLEX series for use in monocots
Schünmann, Petra H. D., Surin, Brian, & Waterhouse, Peter M. (2003) A suite of novel promoters and terminators for plant biotechnology II. The pPLEX series for use in monocots. Functional Plant Biology, 30(4), pp. 453-460.
A suite of plant expression vectors (pPLEX), constructed from the gene regulation signals from subterranean clover stunt virus (SCSV) genome, has previously been used in dicot transformation for a variety of applications in plant biotechnology. To assess their use for the transformation of monocots, a number of modifications were made to the basic vector series and assessed in rice. In their unmodified forms, the SCSV promoters directed low levels of gene expression, however, insertion of an intron between the promoter and the transgene open reading frame (analogous to the rice actin and maize ubiquitin promoter systems) increased transgene expression 50-fold. The expression patterns from the intron-modified SCSV (segments 4 and 7) promoters were very similar to those directed by the actin or ubiquitin promoters. All promoter systems investigated directed expression that appeared to be constitutive within leaf tissue, and localised to the epidermal and vascular tissues of the root. The pPLEX vectors described here are an important counterpart to the dicot pPLEX series and have the potential to be useful in monocot research and biotechnology.
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|Item Type:||Journal Article|
|Keywords:||Actin, Gene expression vectors, Intron, Nanovirus, Subterranean clover stunt virus (SCSV), Ubiquitin, Biotechnology, Genes, Proteins, Tissue, Terminators, Plants (botany), crop improvement, gene expression, Embryophyta, insertion sequences, Liliopsida, Subterranean clover stunt virus, Trifolium, Trifolium subterraneum, vectors, Zea mays|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2003 C S I R O Publishing|
|Deposited On:||09 Jan 2014 04:26|
|Last Modified:||09 Jan 2014 04:27|
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