A suite of novel promoters and terminators for plant biotechnology

Schünmann, Petra H. D., Llewellyn, Danny J., Surin, Brian, Boevink, Petra, De Feyter, Robert C., & Waterhouse, Peter M. (2003) A suite of novel promoters and terminators for plant biotechnology. Functional Plant Biology, 30(4), pp. 443-452.

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The gene regulation signals from subterranean clover stunt virus (SCSV) were investigated for their expression in dicot plants. The SCSV genome has at least eight circular DNA molecules. Each circular DNA component contains a promoter element, a single open reading frame and a terminator. The promoters from seven of the segments were examined for their strength and tissue specificity in transgenic tobacco (Nicotiana tabacum L.), potato (Solanum tuberosum L.) and cotton (Gossypium hirsutum L.) using a GUS reporter gene assay system. While the promoters of many of the segments were poorly expressed, promoters derived from segments 4 and 7 were shown to direct high levels of expression in various plant tissues and organs. The segment 1 promoter directs predominantly callus-specific expression and, when used to control a selectable marker gene, facilitated the transformation of all three species (tobacco, potato and cotton). From the results, a suite of plant expression vectors (pPLEX) derived from the SCSV genome were constructed and used here to produce herbicide- and insect-resistant cotton, demonstrating their utility in the expression of foreign genes in dicot crop species and their potential for use in agricultural biotechnology.

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37 citations in Scopus
28 citations in Web of Science®
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ID Code: 65957
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Bacillus thuringiensis, Gene expression vectors, Herbicide-resistant cotton, Nanovirus, PPLEX, Subterranean clover stunt virus (SCSV), Assays, Biotechnology, DNA, Genes, Molecules, Terminators, Plants (botany), crop improvement, gene expression, pest resistance, Embryophyta, Gossypium, Gossypium hirsutum, Insecta, Nicotiana, Nicotiana tabacum, Solanum, Solanum tuberosum, Subterranean clover stunt virus, Trifolium, Trifolium subterraneum, vectors
DOI: 10.1071/FP02166
ISSN: 1445-4408
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2003 C S I R O Publishing
Deposited On: 09 Jan 2014 04:46
Last Modified: 09 Jan 2014 04:48

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