Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes

Wang, Ming-Bo, Abbott, D. C., Upadhyaya, N. M., Jacobsen, J. V., & Waterhouse, Peter M. (2001) Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes. Australian Journal of Plant Physiology, 28(2), pp. 149-156.

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Abstract

Efficient transformation of barley cv. Schooner was achieved using Agrobacterium delivery, hygromycin or bialaphos selection and embryogenic callus. Using this system, transgenic plants were generated that contained either the green fluorescent protein gene, or transgenes derived from barley yellow dwarf (BYDV) and cereal yellow dwarf (CYDV) viruses. Many of these plants contained 1-3 transgene copies that were inherited in a simple Mendelian manner. Some plants containing BYDV and/or CYDV derived transgenes showed reduced virus symptoms and rates of viral replication when challenged with the appropriate virus. The ability to transform Schooner is a significant advance for the Australian barley industry, as this elite malting variety is, and has for the last 15 years been, the most widely grown barley variety in eastern Australia.

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27 citations in Web of Science®

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ID Code: 65978
Item Type: Journal Article
Refereed: No
Keywords: Bar, BYDV, GFP, Hpt, Schooner, Australia, barley yellow dwarf virus, barley, cereal yellow dwarf virus, cultivar, genetic transformation, inheritance, plant variety, transgene, transgenic plant, virus resistance
ISSN: 0310-7841
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2001 Commonwealth Scientific and Industrial Research Organization
Deposited On: 09 Jan 2014 07:28
Last Modified: 13 Jan 2014 00:48

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