Rice ragged stunt oryzavirus genome segment S4 could encode an RNA dependent RNA polymerase and a second protein of unknown function

Upadhyaya, N. M., Ramm, K., Gellatly, J. A., Li, Z., Kositratana, W., & Waterhouse, Peter M. (1998) Rice ragged stunt oryzavirus genome segment S4 could encode an RNA dependent RNA polymerase and a second protein of unknown function. Archives of Virology, 143(9), pp. 1815-1822.

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Abstract

The complete nucleotide sequence of genome segment S4 of rice ragged stunt oryzavirus (RRSV, Thai-isolate) was determined. The 3823 bp sequence contains two large open reading frames (ORFs). ORF1, spanning nucleotides 12 to 3776, is capable of encoding a protein of M(r) 141,380 (P4a). The P4a amino acid sequence predicted from the nucleotide sequence contains sequence motifs conserved in RNA-dependent RNA polymerases (RDRPs). When compared for evolutionary relationships with RDRPs of other reoviruses using the amino acid sequences around the conserved GDD motif, P4a was shown to be more related to Nilaparvata lugens reovirus and reovirus serotype 3 than to rice dwarf phytoreovirus, bovine rotavirus or bluetongue virus. The ORF2, spanning nucleotides 491 to 1468, is out of frame with ORF1 and is capable of encoding a protein of 36, 920 (P4b). Coupled in vitro transcription-translation from cloned ORF2 in wheat germ extract confirmed the existence of ORF2 but in vivo production and possible function of P4b is yet to be determined.

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ID Code: 66004
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: bluetongue orbivirus, bovine rotavirus, genetic analysis, Nilaparvata lugens, reovirus, rice dwarf phytoreovirus, rice ragged stunt oryzavirus, RNA polymerase, Amino Acid Sequence, Base Sequence, Genome, Viral, Molecular Sequence Data, Open Reading Frames, Oryza sativa, Reoviridae, RNA Replicase, Viral Proteins, Bluetongue virus, Bovinae, Nilaparvata lugens reovirus, Orthoreovirus, Reovirus sp., Rice dwarf virus, Rice ragged stunt virus, Rotavirus, Triticum aestivum
DOI: 10.1007/s007050050419
ISSN: 0304-8608
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1998 Springer Wien
Deposited On: 13 Jan 2014 00:59
Last Modified: 13 Jan 2014 00:59

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