A rapid and simple method of assaying plants transformed with hygromycin or PPT resistance genes
Wang, Ming-Bo & Waterhouse, Peter M. (1997) A rapid and simple method of assaying plants transformed with hygromycin or PPT resistance genes. Plant Molecular Biology Reporter, 15(3), pp. 209-215.
A very simple leaf assay is described that rapidly and reliably identifies transgenic plants expressing the hygromycin resistance gene, hph or the phosphinothricin resistance gene, bar. Leaf tips were cut from plants propagated either in the glasshouse or in tissue culture and the cut surface embedded in solid medium containing the appropriate selective agent. Non-transgenic barley or rice leaf tips had noticeable symptoms of either bleaching or necrosis after three days on the medium and were completely bleached or necrotic after one week. Transgenic leaf tips remained green and healthy over this period. This gave unambiguous discrimination between transgenic and non-transgenic plants. The leaf assay was also effective for dicot plants tested (tobacco and peas).
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|Item Type:||Journal Article|
|Keywords:||Cereals, Leaf assay, Selectable marker, Transformation|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 1997 Springer New York LLC|
|Deposited On:||13 Jan 2014 01:49|
|Last Modified:||13 Jan 2014 01:49|
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