Rice ragged stunt oryzavirus genome segments S7 and S10 encode non-structural proteins of M(r) 68 025 (Pns7) and M(r) 32364 (Pns10) : brief report

Upadhyaya, N. M., Ramm, K., Gellatly, J. A., Li, Z., Kositratana, W., & Waterhouse, Peter M. (1997) Rice ragged stunt oryzavirus genome segments S7 and S10 encode non-structural proteins of M(r) 68 025 (Pns7) and M(r) 32364 (Pns10) : brief report. Archives of Virology, 142(8), pp. 1719-1726.

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Abstract

The nucleotide sequences of genome segments S7 and S10 of a Thai-isolate of rice ragged stunt virus (RRSV) were determined. The 1938 bp S7 sequence contains a single large open reading frame (ORF) spanning nucleotides 20 to 1 843 that is predicted to encode a protein of M(r) 68 025. The 1 162 bp S10 sequence has a major ORF spanning nucleotides 142 to 1 032 that is predicted to encode a protein of M(r) 32364. This S10 ORF is preceded by a small ORF (nt 20-55) which is probably a minicistron. Coupled in vitro transcription-translation from the two major ORFs gave protein products of the expected sizes. However, no protein was visualised from S10 when the small ORF sequence was included. Proteins were expressed in Escherichia coli from the full length ORF of S7 (P7) and from a segment of the S10 ORF (P10) fused to the ORF of glutathione S-transferase (GST). Neither fusion protein was recognised by polyclonal antibodies raised against RRSV particles. Furthermore, polyclonal antibodies raised against GST-P7 fusion protein did not recognise any virion structural polypeptides. These data strongly suggest that the proteins P7 and P10 do not form part of RRSV particle. This is further supported by observed sequence homology (though very weak) of predicted.

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ID Code: 66007
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: S10 protein, Rice ragged stunt virus, S7 protein, Rice ragged stunt virus, virus protein, amino acid sequence, article, genetics, molecular genetics, nucleotide sequence, Reovirus, rice, virology, virus genome, Base Sequence, Genome, Viral, Molecular Sequence Data, Oryza sativa, Reoviridae, Viral Nonstructural Proteins, Escherichia coli, Reovirus sp., Rice ragged stunt virus
DOI: 10.1007/s007050050193
ISSN: 0304-8608
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1997 Springer Wien
Deposited On: 13 Jan 2014 02:17
Last Modified: 13 Jan 2014 02:17

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