Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants
Graham, M. W., Craig, S., & Waterhouse, Peter M. (1997) Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants. Plant Molecular Biology, 33(4), pp. 729-735.
The expression patterns of GUS fusion constructs driven by the Agrobacterium rhizogenes RolC and the maize Sh (Shrunken: sucrose synthase-1) promoters were examined in transgenic potatoes (cv. Atlantic). RolC drove high-level gene expression in phloem tissue, bundle sheath cells and vascular parenchyma, but not in xylem or non-vascular tissues. Sh expression was exclusively confined to phloem tissue. Potato leafroll luteovirus (PLRV) replicates only in phloem tissues, and we show that when RolC is used to drive expression of the PLRV coat protein gene, virus-resistant lines can be obtained. In contrast, no significant resistance was observed when the Sh promoter was used.
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|Item Type:||Journal Article|
|Keywords:||phloem-specific gene expression, potato leafroll virus (PLRV) resistance, RolC promoter, Shrunken promoter, sucrose synthase-1 promoter, vascular-specific gene expression, bacterial protein, beta glucosidase, cytokinin beta glucosidase, cytokinin-beta-glucosidase, glucosyltransferase, hybrid protein, sucrose synthase, animal, aphid, article, biosynthesis, genetic engineering, genetics, innate immunity, Luteovirus, methodology, pathogenicity, plant disease, potato, promoter region, tissue distribution, transgenic plant, virology, virus capsid, Animals, Aphids, Bacterial Proteins, beta-Glucosidase, Capsid, Glucosyltransferases, Immunity, Natural, Plant Diseases, Plants, Genetically Modified, Promoter Regions (Genetics), Recombinant Fusion Proteins, Solanum tuberosum, Agrobacterium rhizogenes, Animalia, Aphididae, Bacteria (microorganisms), Miridae, Potato leafroll virus, Zea mays|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 1997 Springer Netherlands|
|Deposited On:||13 Jan 2014 02:56|
|Last Modified:||13 Jan 2014 02:56|
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