Genome segment 5 of rice ragged stunt virus encodes a virion protein

Li, Z., Upadhyaya, N. M., Kositratana, W., Gibbs, A. J., & Waterhouse, Peter M. (1996) Genome segment 5 of rice ragged stunt virus encodes a virion protein. Journal of General Virology, 77(12), pp. 3155-3160.

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Abstract

The complete nucleotide sequence of the genome segment 5 (S5) of a Thai isolate of rice ragged stunt virus (RRSV) was determined. The 2682 nucleotide sequence contains a single long open reading frame capable of encoding a polypeptide with a molecular mass of ~91 kDa. Polypeptides encoded by various truncated cDNAs of S5 were expressed using the pGEX fusion protein vector and the highest level of fusion protein was obtained from a construct encoding a hydrophilic region of S5 protein. Antibodies raised against this fusion protein recognized a minor polypeptide, with a molecular mass of ~ 91 kDa, that was present in purified preparations of RRSV particles, infected insect vectors and infected rice plants. This indicates that RRSV S5 encodes a minor structural protein. Comparing the RRSV S5 sequence with sequences of other reo-viruses did not reveal any significant sequence similarities.

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11 citations in Scopus
9 citations in Web of Science®
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ID Code: 66011
Item Type: Journal Article
Refereed: Yes
Keywords: complementary DNA, hybrid protein, protein antibody, virus protein, virus vector, animal experiment, animal tissue, antigen recognition, article, controlled study, insect, molecular weight, nonhuman, nucleotide sequence, open reading frame, priority journal, protein expression, Reovirus, virion, virus genome, Amino Acid Sequence, Animals, Base Sequence, Genome, Viral, Molecular Sequence Data, Oryza sativa, Plant Viruses, Rabbits, Reoviridae, RNA, Viral, Viral Structural Proteins, Animalia, Insecta, reo virus, Reovirus sp., Rice ragged stunt virus
ISSN: 0022-1317
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1996 Society for General Microbiology
Deposited On: 13 Jan 2014 04:28
Last Modified: 20 Jul 2017 08:01

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