Characterisation of the subgenomic RNAs of an Australian isolate of barley yellow dwarf luteovirus

Kelly, L., Gerlach, W. L., & Waterhouse, Peter M. (1994) Characterisation of the subgenomic RNAs of an Australian isolate of barley yellow dwarf luteovirus. Virology, 202(2), pp. 565-573.

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Abstract

We have characterised the subgenomic RNAs of an Australian isolate of BYDV-PAV. Northern blot analyses of infected plants and protoplasts have shown that this isolate synthesises three subgenomic RNAs. Precise mapping of the transcription start sites of all three subgenomic RNAs and translational analyses of subgenomic RNA 2 and 3 have revealed a number of features. First, the transcription start site of subgenomic RNA 1 in this isolate differs markedly from the start site determined for an Illinois isolate of BYDV-PAV. Second, the start sites of subgenomic RNA 1 and 2 occur at a sequence that closely resembles the 5' end sequence of the genomic RNA (5'AGUGAAGA). Third, subgenomic RNA 2 appears to express ORF 6 of BYDV-PAV but the gene product is truncated due to the appearance of a new stop codon in the sequence. Last, subgenomic RNA 3, which is abundantly transcribed and encapsidated by the virus particle, appears to have no coding ability. We postulate that this novel subgenomic RNA has a regulatory function.

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ID Code: 66108
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: gene product, ribonuclease, virus rna, article, codon, molecular cloning, nonhuman, northern blotting, oat, plant virus, plasmid, priority journal, protoplast, rna sequence, transcription initiation, virus isolation, virus transcription, wheat, Australia, Base Sequence, Cloning, Molecular, DNA Primers, Gene Expression Regulation, Viral, Genes, Structural, Viral, Hordeum, Luteovirus, Molecular Sequence Data, RNA, Messenger, RNA, Viral, Support, Non-U.S. Gov't, Transcription, Genetic, Translation, Genetic, Barley yellow dwarf virus, Hordeum vulgare subsp. vulgare, RNA viruses, Triticum aestivum
DOI: 10.1006/viro.1994.1378
ISSN: 0042-6822
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1994 Academic Press
Deposited On: 13 Jan 2014 06:22
Last Modified: 13 Jan 2014 06:22

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