Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein

Chu, Paul W. G., Paul, Keese, Qiu, Bing-Sheng, Waterhouse, Peter M., & Gerlach, Wayne L. (1993) Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. Virus Research, 27(2), pp. 161-171.

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Abstract

Subterranean clover stunt disease is an economically important aphid-borne virus disease affecting certain pasture and grain legumes in Australia. The virus associated with the disease, subterranean clover stunt virus (SCSV), was previously found to be representative of a new type of single-stranded DNA virus. Analysis of the virion DNA and restriction mapping of double-stranded cDNA synthesized from virion DNA suggested that SCSV has a segmented genome composed of 3 or 4 different species of circular ssDNA each of about 850-880 nucleotides. To further investigate the complexity of the SCSV genome, we have isolated the replicative form DNA from infected pea and from it prepared putative full-length clones representing the SCSV genome segments. Analysis of these clones by restriction mapping indicated that clones representing at least 4 distinct genomic segments were obtained. This method is thus suitable for generating an extensive genomic library of novel ssDNA viruses containing multiple genome segments such as SCSV and banana bunchy top virus. The N-terminal amino acid sequence and amino acid composition of the coat protein of SCSV were determined. Comparison of the amino acid sequence with partial DNA sequence data, and the distinctly different restriction maps obtained for the full-length clones suggested that only one of these clones contained the coat protein gene. The results confirmed that SCSV has a functionally divided genome composed of several distinct ssDNA circles each of about 1 kb.

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ID Code: 66112
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Coat protein, Full-length clone, N-terminal amino acid sequence, Replicative form, Subterranean clover stunt virus, virus capsid antigen, amino acid sequence, article, gene expression, nonhuman, plant virus, priority journal, restriction mapping, virus envelope, virus gene, Base Sequence, Capsid, Cloning, Molecular, DNA, Viral, Genome, Viral, Molecular Sequence Data, Plant Viruses, Support, Non-U.S. Gov't, Aphididae, Banana bunchy top virus, DNA viruses, Miridae, Pisum sativum, ssDNA viruses, Trifolium subterraneum
DOI: 10.1016/0168-1702(93)90079-3
ISSN: 0168-1702
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1993 Elsevier BV
Deposited On: 13 Jan 2014 07:06
Last Modified: 28 Apr 2015 04:29

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