Infectious in vitro transcripts from a cloned cDNA of barley yellow dwarf virus

Young, M. J., Kelly, L., Larkin, P. J., Waterhouse, Peter M., & Gerlach, W. L. (1991) Infectious in vitro transcripts from a cloned cDNA of barley yellow dwarf virus. Virology, 180(1), pp. 372-379.

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Abstract

A full-length cDNA clone of barley yellow dwarf virus (BYDV-PAV serotype) has been constructed and fused to the bacteriophage T7 RNA polymerase promoter. RNA transcripts produced in vitro, either capped or uncapped, were infectious in Triticum monococcum protoplasts. Protoplasts inoculated with in vitro-transcribed BYDV RNA accumulated coat protein, synthesized new viral RNAs, and produced virus particles. Aphid feeding on extracts from protoplasts inoculated with in vitro RNA transcripts can be used to transfer the virus progeny to whole plants. Introduction of mutations which interrupt specific BYDV-PAV open reading frames (ORFs) V and VI eliminated infectivity while an ORF I mutant remained infectious. Infectious RNA transcripts derived from BYDV cDNA clones will facilitate analysis of the molecular aspects of BYDV infection and further enhance our understanding of this economically important virus.

Impact and interest:

28 citations in Scopus
47 citations in Web of Science®
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ID Code: 66167
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: complementary dna, article, barley, molecular cloning, nonhuman, plant virus, priority journal, virus characterization, virus infectivity, virus transcription, Animal, Aphids, Base Sequence, Cloning, Molecular, DNA, Viral, DNA-Directed RNA Polymerases, Frameshift Mutation, Genes, Viral, Hordeum, In Vitro, Molecular Sequence Data, Plant Diseases, Plant Viruses, Promoter Regions (Genetics), Protoplasts, RNA, Viral, Support, Non-U.S. Gov't, T-Phages, Transcription, Genetic, Translation, Genetic, Aphididae, Barley yellow dwarf virus, Hordeum vulgare subsp. vulgare, Triticum monococcum
DOI: 10.1016/0042-6822(91)90042-A
ISSN: 0042-6822
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1991 Academic Press
Deposited On: 14 Jan 2014 06:03
Last Modified: 14 Jan 2014 06:04

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