Sequence and identification of the barley yellow dwarf virus coat protein gene

Miller, W. A., Waterhouse, Peter M., Kortt, A. A., & Gerlach, W. L. (1988) Sequence and identification of the barley yellow dwarf virus coat protein gene. Virology, 165(1), pp. 306-309.

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Abstract

The nucleotide sequence of the coat protein gene of barley yellow dwarf virus (BYDV, PAV serotype) was determined, and the amino acid sequence was deduced. The open reading frame, encoding a protein of relative molecular mass (Mr) 22,047, was confirmed as the coat protein gene by comparison with amino acid sequences of tryptic peptides derived from dissociated virions. In addition, a fragment of this gene expressed in Escherichia coli produced a product which was recognized by antibodies prepared against purified BYDV virions. An overlapping reading frame encoding an Mr 17,147 protein is contained completely within the coat protein gene. © 1988.

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ID Code: 66170
Item Type: Journal Article
Refereed: Yes
Keywords: DNA, hybrid protein, virus antibody, amino acid sequence, article, genetics, immunology, molecular genetics, nucleotide sequence, plant virus, virus capsid, virus gene, Antibodies, Viral, Base Sequence, Capsid, Genes, Viral, Molecular Sequence Data, Plant Viruses, Recombinant Fusion Proteins, Support, Non-U.S. Gov't
ISSN: 0042-6822
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1988 Academic Press
Deposited On: 14 Jan 2014 06:15
Last Modified: 14 Jan 2014 06:15

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