Carrot red leaf and carrot mottle viruses : observations on the composition of the particles in single and mixed infections

Murant, A. F., Waterhouse, Peter M., Raschke, J. H., & Robinson, D. J. (1985) Carrot red leaf and carrot mottle viruses : observations on the composition of the particles in single and mixed infections. Journal of General Virology, 66(7), pp. 1575-1579.

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Abstract

Particles of carrot red leaf virus (CRLV; luteovirus group) purified from chervil (Anthriscus cerefolium) contain a single ssRNA species of mol. wt. about 1.8 x 106 and a major protein of mol. wt. about 25000. CRLV acts as a helper for aphid transmission of carrot mottle virus (CMotV; ungrouped) from mixedly infected plants. Virus preparations purified from such plants possess the infectivity of both viruses but contain particles indistinguishable from those of CRLV; some of the particles are therefore thought to consist of CMotV RNA packaged in CRLV coat protein. When RNA from such preparations was electrophoresed in agarose/polyacrylamide gels, CMotV infectivity was associated with an RNA band that migrated ahead of the CRLV RNA band and had an estimated mol. wt. of about 1.5 x 106, similar to that previously found for the infective ssRNA extracted directly from Nicotiana clevelandii leaves infected with CMotV alone. Preparations of dsRNA from CMotV-infected N. clevelandii leaves contained two species: one of mol. wt. about 3.2 x 106, presumably the replicative form of the infective ssRNA, and the other, mol. wt. about 0.9 x 106, of unknown origin and function. The infective agent in buffer extracts of CMotV-infected N. clevelandii was resistant to RNase (although the enzyme acted as a reversible inhibitor of infection at high concentrations) and is therefore not unprotected RNA. It may be protected within the approximately 52 nm enveloped structures previously reported.

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ID Code: 66173
Item Type: Journal Article
Refereed: Yes
Keywords: dodecyl sulfate, virus protein, virus rna, agar gel electrophoresis, higher plant, nonhuman, plant virus, polyacrylamide gel electrophoresis, priority journal, virus infection
ISSN: 0022-1317
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1985 Society for General Microbiology
Deposited On: 14 Jan 2014 06:42
Last Modified: 14 Jan 2014 06:42

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