Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase
Waterhouse, Peter M. & Helms, K. (1984) Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase. Journal of Virological Methods, 8(4), pp. 321-329.
Particles of two isolates of subterranean clover red leaf virus were purified by a method in which infected plant tissue was digested with an industrial-grade cellulase, Celluclast® 2.0 L type X. The yields of virus particles using this enzyme were comparable with those obtained using either of two laboratory-grade cellulases, Cellulase type 1 (Sigma) and Driselase®. However, the specific infectivity or aphid transmissibility of the particles purified using Celluclast® was 10-100 times greater than those of preparations obtained using laboratory-grade cellulases or no enzyme. The main advantage of using Celluclast® is that at present in Australia its cost is only ca. 1% of laboratory-grade cellulases.
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|Item Type:||Journal Article|
|Keywords:||clover red leaf virus, enzyme immunoassay, higher plant, nonhuman, virus purification, Animal, Aphids, Cellulase, Comparative Study, Enzyme-Linked Immunosorbent Assay, Fabaceae, Glycoside Hydrolases, Plant Diseases, Plant Viruses, Plants, Medicinal|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 1984 Elsevier BV|
|Deposited On:||14 Jan 2014 06:45|
|Last Modified:||14 Jan 2014 06:45|
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