Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase

Waterhouse, Peter M. & Helms, K. (1984) Purification of particles of subterranean clover red leaf virus using an industrial-grade cellulase. Journal of Virological Methods, 8(4), pp. 321-329.

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Abstract

Particles of two isolates of subterranean clover red leaf virus were purified by a method in which infected plant tissue was digested with an industrial-grade cellulase, Celluclast® 2.0 L type X. The yields of virus particles using this enzyme were comparable with those obtained using either of two laboratory-grade cellulases, Cellulase type 1 (Sigma) and Driselase®. However, the specific infectivity or aphid transmissibility of the particles purified using Celluclast® was 10-100 times greater than those of preparations obtained using laboratory-grade cellulases or no enzyme. The main advantage of using Celluclast® is that at present in Australia its cost is only ca. 1% of laboratory-grade cellulases.

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21 citations in Web of Science®

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ID Code: 66174
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: clover red leaf virus, enzyme immunoassay, higher plant, nonhuman, virus purification, Animal, Aphids, Cellulase, Comparative Study, Enzyme-Linked Immunosorbent Assay, Fabaceae, Glycoside Hydrolases, Plant Diseases, Plant Viruses, Plants, Medicinal
DOI: 10.1016/0166-0934(84)90069-7
ISSN: 0166-0934
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 1984 Elsevier BV
Deposited On: 14 Jan 2014 06:45
Last Modified: 14 Jan 2014 06:45

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