The effect of nitroxides on swarming motility and biofilms, multicellular behaviors in Pseudomonas aeruginosa

de la Fuente-Núñez, César, Reffuveille , Fany, Fairfull-Smith, Kathryn, & Hancock, Robert (2013) The effect of nitroxides on swarming motility and biofilms, multicellular behaviors in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 57(10), pp. 4877-4881.

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The ability of NO to induce biofilm dispersion has been well established. Here we investigated the effect of nitroxides (sterically hindered nitric oxide analogues) on biofilm formation and swarming motility in Pseudomonas aeruginosa. A transposon mutant unable to produce nitric oxide endogenously (nirS) was deficient in swarming motility relative to wild type and the complemented strain. Moreover, expression of the nirS gene was up-regulated by 9.65-fold in wild type swarming cells when compared to planktonic cells. Wild type swarming levels were substantially restored upon exogenous addition of nitroxide containing compounds, consistent with the hypothesis that NO is necessary for swarming motility. Here, we showed that nitroxides not only mimicked the dispersal activity of NO, but also prevented biofilms from forming in flow cell chambers. In addition, a nirS transposon mutant was deficient in biofilm formation relative to wild type and the complemented strain, thus implicating NO in the formation of biofilms. Intriguingly despite its stand alone action in inhibiting biofilm formation and promoting dispersal, a nitroxide partially restored the ability of a nirS mutant to form biofilms.

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16 citations in Scopus
12 citations in Web of Science®
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ID Code: 66650
Item Type: Journal Article
Refereed: Yes
DOI: 10.1128/AAC.01381-13
ISSN: 0066-4804
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MEDICINAL AND BIOMOLECULAR CHEMISTRY (030400) > Biologically Active Molecules (030401)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 American Society for Microbiology
Deposited On: 28 Jan 2014 22:24
Last Modified: 11 Apr 2014 01:38

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