Validation of β-actin used as endogenous control for gene expression analysis in mechanobiology studies : amendments
Kasper, Grit, Ode, Andrea, Groothuis, Aline, Glaeser, Juliane, Gaber, Timo, Wilson, Cameron J., Geissler, Sven, & Duda, Georg N. (2010) Validation of β-actin used as endogenous control for gene expression analysis in mechanobiology studies : amendments. Stem Cells, 28(3), pp. 633-634.
We write in response to the letter by Liu et al.  commenting on our article, ‘‘Mesenchymal Stem Cells Regulate Angiogenesis According to Their Mechanical Environment’’ . The study by Liu et al. demonstrates that the commonly used endogeneous reference gene (ERG), b-actin, is upregulated by mechanical loading, indicating a potential bias in the determined target gene expression when normalizing to b-actin, such as in our report on unchanged vascular endothelial growth factor (VEGF) and hypoxia-inducible factors (HIF)-1a mRNA levels in mechanically loaded mesenchymal stem cells (MSCs).
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|Item Type:||Journal Article|
|Keywords:||gene expression, mechanotransduction|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Metabolism (060104)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomechanical Engineering (090302)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL PHYSIOLOGY (111600) > Cell Physiology (111601)
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Deposited On:||07 Feb 2014 01:16|
|Last Modified:||27 Sep 2015 23:59|
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