Validation of β-actin used as endogenous control for gene expression analysis in mechanobiology studies : amendments

Kasper, Grit, Ode, Andrea, Groothuis, Aline, Glaeser, Juliane, Gaber, Timo, Wilson, Cameron J., Geissler, Sven, & Duda, Georg N. (2010) Validation of β-actin used as endogenous control for gene expression analysis in mechanobiology studies : amendments. Stem Cells, 28(3), pp. 633-634.

View at publisher

Abstract

We write in response to the letter by Liu et al. [1] commenting on our article, ‘‘Mesenchymal Stem Cells Regulate Angiogenesis According to Their Mechanical Environment’’ [2]. The study by Liu et al. demonstrates that the commonly used endogeneous reference gene (ERG), b-actin, is upregulated by mechanical loading, indicating a potential bias in the determined target gene expression when normalizing to b-actin, such as in our report on unchanged vascular endothelial growth factor (VEGF) and hypoxia-inducible factors (HIF)-1a mRNA levels in mechanically loaded mesenchymal stem cells (MSCs).

Impact and interest:

5 citations in Scopus
Search Google Scholar™
3 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 67011
Item Type: Journal Article
Refereed: Yes
Keywords: gene expression, mechanotransduction
DOI: 10.1002/stem.305
ISSN: 1066-5099
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Metabolism (060104)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomechanical Engineering (090302)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL PHYSIOLOGY (111600) > Cell Physiology (111601)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 07 Feb 2014 01:16
Last Modified: 27 Sep 2015 23:59

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page