A porcine deep dermal partial thickness burn model with hypertrophic scarring

Cuttle, Leila, Kempf, M., Phillips, G. E., Mill, J., Hayes, M. T., Fraser, J. F., Wang, X. Q., & Kimble, R. M. (2006) A porcine deep dermal partial thickness burn model with hypertrophic scarring. Burns, 32(7), pp. 806-820.

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We developed a reproducible model of deep dermal partial thickness burn injury in juvenile Large White pigs. The contact burn is created using water at 92 degrees C for 15s in a bottle with the bottom replaced with plastic wrap. The depth of injury was determined by a histopathologist who examined tissue sections 2 and 6 days after injury in a blinded manner. Upon creation, the circular wound area developed white eschar and a hyperaemic zone around the wound border. Animals were kept for 6 weeks or 99 days to examine the wound healing process. The wounds took between 3 and 5 weeks for complete re-epithelialisation. Most wounds developed contracted, purple, hypertrophic scars. On measurement, the thickness of the burned skin was approximately 1.8 times that of the control skin at week 6 and approximately 2.2 times thicker than control skin at 99 days after injury. We have developed various methods to assess healing wounds, including digital photographic analysis, depth of organising granulation tissue, immunohistochemistry, electron microscopy and tensiometry. Immunohistochemistry and electron microscopy showed that our porcine hypertrophic scar appears similar to human hypertrophic scarring. The development of this model allows us to test and compare different treatments on burn wounds.

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ID Code: 67197
Item Type: Journal Article
Refereed: Yes
Additional Information: Cuttle, Leila Kempf, Margit Phillips, Gael E Mill, Julie Hayes, Mark T Fraser, John F Wang, Xue-Qing Kimble, Roy M eng Research Support, Non-U.S. Gov't England 2006/08/04 09:00 Burns. 2006 Nov;32(7):806-20. Epub 2006 Aug 1.
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Keywords: Animals, Burns/*pathology, Cicatrix, Hypertrophic/*pathology, Collagen/ultrastructure, Immunohistochemistry, Microscopy, Electron, Scanning, Models, Animal, Skin/*injuries/ultrastructure, Swine
DOI: 10.1016/j.burns.2006.02.023
ISSN: 1879-1409 (online) 0305-4179 (Print)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright © 2014 Elsevier B.V. except certain content provided by third parties. ScienceDirect® is a registered trademark of Elsevier B.V.
Deposited On: 26 Feb 2014 23:12
Last Modified: 26 Mar 2014 00:05

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