Collagen in the scarless fetal skin wound : detection with picrosirius-polarization

Cuttle, Leila, Nataatmadja, M., Fraser, J. F., Kempf, M., Kimble, R. M., & Hayes, M. T. (2005) Collagen in the scarless fetal skin wound : detection with picrosirius-polarization. Wound Repair and Regeneration, 13(2), pp. 198-204.

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Our group has developed an ovine model of deep dermal, partial-thickness burn where the fetus heals scarlessly and the lamb heals with scar. The comparison of collagen structure between these two different mechanisms of healing may elucidate the process of scarless wound healing. Picrosirius staining followed by polarized light microscopy was used to visualize collagen fibers, with digital capture and analysis. Collagen deposition increased with fetal age and the fibers became thicker, changing from green (type III collagen) to yellow/red (type I collagen). The ratio of type III collagen to type I was high in the fetus (166), whereas the lamb had a much lower ratio (0.2). After burn, the ratios of type III to type I collagen did not differ from those in control skin for either fetus or lamb. The fetal tissue maintained normal tissue architecture after burn while the lamb tissue showed irregular collagen organization. In conclusion, the type or amount of collagen does not alter significantly after injury. Tissue architecture differed between fetal and lamb tissue, suggesting that scar development is related to collagen cross-linking or arrangement. This study indicates that healing in the scarless fetal wound is representative of the normal fetal growth pattern, rather than a "response" to burn injury.

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ID Code: 67201
Item Type: Journal Article
Refereed: Yes
Additional Information: Cuttle, Leila
Nataatmadja, Maria
Fraser, John F
Kempf, Margit
Kimble, Roy M
Hayes, Mark T
Research Support, Non-U.S. Gov't
2005/04/15 09:00
Wound Repair Regen. 2005 Mar-Apr;13(2):198-204.
Additional URLs:
Keywords: Animals, Azo Compounds/diagnostic use, Cicatrix/*pathology/physiopathology, Collagen/*physiology, Coloring Agents, Fetus, Microscopy, Polarization/*methods, Models, Animal, Sheep, Skin/*pathology/physiopathology, Wounds and Injuries/*pathology/physiopathology
DOI: 10.1111/j.1067-1927.2005.130211.x
ISSN: 1067-1927 (print) 1524-475X (online)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2005 The Wound Healing Society
Deposited On: 27 Feb 2014 01:23
Last Modified: 25 Mar 2014 23:23

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