Interleukin-1β stimulates human renal fibroblast proliferation and matrix protein production by means of a transforming growth factor-β-dependent mechanism

Vesey, David A., Cheung, Cathrine, Cuttle, Leila, Endre, Zoltan, Gobe, Glenda, & Johnson, David W. (2002) Interleukin-1β stimulates human renal fibroblast proliferation and matrix protein production by means of a transforming growth factor-β-dependent mechanism. Journal of Laboratory and Clinical Medicine, 140(5), pp. 342-350.

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One of the hallmarks of progressive renal disease is the development of tubulointerstitial fibrosis. This is frequently preceded by macrophage infiltration, raising the possibility that macrophages relay fibrogenic signals to resident tubulointerstitial cells. The aim of this study was to investigate the potentially fibrogenic role of interleukin-1beta (IL-1beta), a macrophage-derived inflammatory cytokine, on cortical fibroblasts (CFs). Primary cultures of human renal CFs were established and incubated for 24 hours in the presence or absence of IL-1beta. We found that IL-1beta significantly stimulated DNA synthesis (356.7% +/- 39% of control, P <.003), fibronectin secretion (261.8 +/- 11% of control, P <.005), collagen type 1 production, (release of procollagen type 1 C-terminal-peptide, 152.4% +/- 26% of control, P <.005), transforming growth factor-beta (TGF-beta) secretion (211% +/- 37% of control, P <.01), and nitric oxide (NO) production (342.8% +/- 69% of control, P <.002). TGF-beta (1 ng/mL) and the phorbol ester phorbol 12-myristate 13-acetate (PMA, 25 nmol/L) produced fibrogenic effects similar to those of IL-1beta. Neither a NO synthase inhibitor (N(G)-methyl-l-arginine, 1 mmol/L) nor a protein kinase C (PKC) inhibitor (bis-indolylmaleimide 1, 1 micromol/L) altered the enhanced level of fibronectin secretion or DNA synthesis seen in response to IL-1beta treatment. However, addition of a TGF-beta-neutralizing antibody significantly reduced IL-1beta-induced fibronectin secretion (IL-1beta + IgG, 262% +/- 72% vs IL-1beta + alphaTGF-beta 156% +/- 14%, P <.02), collagen type 1 production (IL-1beta + IgG, 176% +/- 28% vs IL-1beta + alphaTGF-beta, 120% +/- 14%, P <.005) and abrogated IL-1beta-induced DNA synthesis (245% +/- 49% vs 105% +/- 21%, P <.005). IL-1beta significantly stimulated CF DNA synthesis and production of fibronectin, collagen type 1, TGFbeta, and NO. The fibrogenic and proliferative action of IL-1beta on CF appears not to involve activation of PKC or production of NO but is at least partly TGFbeta-dependent.

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ID Code: 67219
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Antibodies/immunology/pharmacology, Cells, Cultured, Collagen Type I/metabolism, DNA/biosynthesis, DNA Replication/drug effects, Dose-Response Relationship, Drug, Enzyme Inhibitors/pharmacology, Extracellular Matrix Proteins/*drug effects/metabolism, Female, Fibroblasts/cytology/*drug effects/metabolism, Fibronectins/metabolism, Humans, Interleukin-1/*pharmacology, Kidney Cortex/cytology/*drug effects/metabolism, Male, Nitric Oxide/metabolism, Tetradecanoylphorbol Acetate/pharmacology, *Transforming Growth Factor beta/immunology/metabolism
DOI: 10.1067/mlc.2002.128468
ISSN: 0022-2143
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 Elsevier
Copyright Statement: This is the author’s version of a work that was accepted for publication in Journal of Laboratory and Clinical Medicine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Laboratory and Clinical Medicine, [VOL 140, ISSUE 50, (2002)] DOI: 10.1067/mlc.2002.128468
Deposited On: 26 Feb 2014 23:43
Last Modified: 26 Feb 2014 23:43

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