Controlling whole blood activation and resultant clot properties by carboxyl and alkyl functional groups on material surfaces : a possible therapeutic approach for enhancing bone healing
Shiu, Hoi Ting, Goss, Ben, Lutton, Cameron, Crawford, Ross W., & Xiao, Yin (2014) Controlling whole blood activation and resultant clot properties by carboxyl and alkyl functional groups on material surfaces : a possible therapeutic approach for enhancing bone healing. Journal of Materials Chemistry B, 2(20), pp. 3009-3021.
Most research virtually ignores the important role of a blood clot in supporting bone healing. In this study, we investigated the effects of surface functional groups carboxyl and alkyl on whole blood coagulation, complement activation and blood clot formation. We synthesised and tested a series of materials with different ratios of carboxyl (–COOH) and alkyl (–CH3, –CH2CH3 and –(CH2)3CH3) groups. We found that surfaces with –COOH/–(CH2)3CH3 induced a faster coagulation activation than those with –COOH/– CH3 and –CH2CH3, regardless of the –COOH ratios. An increase in –COOH ratios on –COOH/–CH3 and –CH2CH3 surfaces decreased the rate of coagulation activation. The pattern of complement activation was entirely similar to that of surface-induced coagulation. All material coated surfaces resulted in clots with thicker fibrin in a denser network at the clot/material interface and a significantly slower initial fibrinolysis when compared to uncoated glass surfaces. The amounts of platelet-derived growth factor-AB (PDGF-AB) and transforming growth factor-b (TGF-b1) released from an intact clot were higher than a lysed clot. The release of PDGF-AB was found to be correlated with the fibrin density. This study demonstrated that surface chemistry can significantly influence the activation of blood coagulation and complement system, resultant clot structure, susceptibility to fibrinolysis as well as release of growth factors, which are important factors determining the bone healing process.
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|Item Type:||Journal Article|
|Keywords:||biomaterials, carboxyl, alkyl, coagulation, bone, clot|
|ISSN:||2050-7518 (online) 2050-750X (print)|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
|Divisions:||Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2014 The Royal Society of Chemistry|
|Copyright Statement:||Author's Pre-print: author can archive pre-print (ie pre-refereeing)
Author's Post-print: author can archive post-print (ie final draft post-refereeing)
Publisher's Version/PDF: author can archive publisher's version/PDF
General Conditions: •Pre-prints on non-commercial repositories and arXiv
•Post-print on author's personal website
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•Publisher's version/PDF may be used on author's personal website only
•Publisher PDF will be supplied and may be used on author's personal website only
•RSC will deposit the authors post-print, if appropriate in non-commercial repositories, not limited to funder's repositories after 12 months•Restrictions on further re-use and further distribution to be noted
|Deposited On:||29 Apr 2014 22:58|
|Last Modified:||05 May 2014 06:10|
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