Epigenetic regulation of chemokine/chemokine receptor expression

Baird, Anne-Marie, O'Byrne, Kenneth J., & Gray , Steven G. (2013) Epigenetic regulation of chemokine/chemokine receptor expression. Methods Mol Biol, 1013, pp. 185-201.

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Abstract

Epigenetic regulation of gene expression is an important event for normal cellular homeostasis. Gene expression may be "switched" on or "turned" off via epigenetic means through adjustments in DNA architecture. These structural alterations result from changes to the DNA methylation status in addition to histone posttranslational modifications such as acetylation and methylation. Drugs which can alter the status of these epigenetic markers are currently undergoing clinical trials in a wide variety of diseases, including cancer.We illustrate the treatment of cell lines with histone deacetylase (HDi) and DNA methyltransferase inhibitors and the subsequent RNA isolation and reverse transcriptase polymerase chain reaction for several members of the CXC (ELR(+)) chemokine family. In addition we describe a chromatin immunoprecipitation assay to determine the association between chromatin transcription markers and DNA following pretreatment of cell cultures with an HDi, Trichostatin A (TSA). This assay allows us to determine whether treatment with TSA dynamically remodels the promoter region of our selected genes, as judged by the differences in the PCR product between our treated and untreated samples.

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ID Code: 67766
Item Type: Journal Article
Refereed: Yes
Keywords: Animals, Cell Culture Techniques, Cell Line, Chemokines, CXC/ genetics/metabolism, Chromatin Immunoprecipitation, DNA Modification Methylases/antagonists & inhibitors/metabolism, Epigenesis, Genetic/drug effects, Gene Expression Regulation, Histone Deacetylase Inhibitors/pharmacology, Humans, Promoter Regions, Genetic, RNA/isolation & purification/metabolism, Receptors, CXCR/ genetics/metabolism, Reverse Transcriptase Polymerase Chain Reaction
DOI: 10.1007/978-1-62703-426-5_12
ISSN: 1940-6029
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2013 Springer
Deposited On: 26 Feb 2014 01:30
Last Modified: 30 Jul 2014 01:56

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