Global analysis of serum microRNAs as potential biomarkers for lung adenocarcinoma

Rani, Sweta, Gately, Kathy, Crown, John, O'Byrne, Ken, & O'Driscoll, Lorraine (2013) Global analysis of serum microRNAs as potential biomarkers for lung adenocarcinoma. Cancer Biology and Therapy, 14(12), pp. 1104-1112.

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Abstract

Early diagnosis and the ability to predict the most relevant treatment option for individuals is essential to improve clinical outcomes for non-small cell lung cancer (NSCLC) patients. Adenocarcinoma (ADC), a subtype of NSCLC, is the single biggest cancer killer and therefore an urgent need to identify minimally invasive biomarkers to enable early diagnosis. Recent studies, by ourselves and others, indicate that circulating miRNA s have potential as biomarkers. Here we applied global profiling approaches in serum from patients with ADC of the lung to explore if miRNA s have potential as diagnostic biomarkers. This study involved RNA isolation from 80 sera specimens including those from ADC patients (equal numbers of stages 1, 2, 3, and 4) and age- and gender-matched controls (n = 40 each). Six hundred and sixty-seven miRNA s were co-analyzed in these specimens using TaqMan low density arrays and qPCR validation using individual miRNA s. Overall, approximately 390 and 370 miRNA s were detected in ADC and control sera, respectively. A group of 6 miRNA s, miR-30c-1* (AU C = 0.74; P < 0.002), miR-616(AU C = 0.71; P = 0.001), miR-146b-3p (AU C = 0.82; P < 0.0001), miR-566 (AU C = 0.80; P < 0.0001), miR-550 (AU C = 0.72; P = 0.0006), and miR-939 (AU C = 0.82; P < 0.0001) was found to be present at substantially higher levels in ADC compared with control sera. Conversely, miR-339-5p and miR-656 were detected at substantially lower levels in ADC sera (co-analysis resulting in AU C = 0.6; P = 0.02). Differences in miRNA profile identified support circulating miRNA s having potential as diagnostic biomarkers for ADC. More extensive studies of ADC and control serum specimens are warranted to independently validate the potential clinical relevance of these miRNA s as minimally invasive biomarkers for ADC.

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ID Code: 67780
Item Type: Journal Article
Refereed: Yes
Keywords: Adenocarcinoma, Biomarker, Circulating, Lung cancer, Micro-RNAs (miRNAs), Minimally invasive, ROC curve, microRNA, microRNA 103, microRNA 146b 3p, microRNA 16, microRNA 192, microRNA 30c 1, microRNA 339 5p, microRNA 451, microRNA 550, microRNA 566, microRNA 616, microRNA 939, tumor marker, unclassified drug, untranslated RNA, age, area under the curve, article, cancer patient, controlled study, density, disease severity, down regulation, female, gender, human, lung adenocarcinoma, major clinical study, male, polymerase chain reaction, receiver operating characteristic, RNA isolation, upregulation
DOI: 10.4161/cbt.26370
ISSN: 1538-4047
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2013 Landes Bioscience
Deposited On: 25 Feb 2014 04:01
Last Modified: 26 Feb 2014 00:08

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