Repeatability of confocal microscopy measurements on the central and peripheral cornea

Makrynioti, Dimitra, Morgan, Philip B., & Efron, Nathan (2010) Repeatability of confocal microscopy measurements on the central and peripheral cornea. Contact Lens & Anterior Eye, 33(6), p. 296.

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To assess confocal microscopy repeatability (ConfoScan3, Nidek, Italy) when assessing the morphology of the limbus, midperipheral and central cornea.


The central, mid-peripheral and limbal cornea (temporal and nasal) of the right eye of 8 subjects were examined with a ConfoScan3 in two different visits, at least six months apart. Bland-Altman repeatability was measured for 29 parameters: basal cell density and size, anterior and posterior keratocyte densities (AKD/PKD), endothelial cell density, polymegethism, pleomorphism, mean area and sides of endothelial cells - in the five different corneal areas examined.


As a percentage of the mean absolute values, repeatability of 0–10% was classified as “excellent”, between 10–30% as “acceptable” and over 30% as “poor”. Repeatability was excellent for 14% of parameters and acceptable for 52% of parameters. The number of endothelial cell sides in the central cornea demonstrated the best repeatability (2.0%) whilst mid-temporal PKD showed the poorest repeatability (53.7%).


Confocal microscopy is at least an adequately repeatablemethodof evaluating the various corneal layers at different locations. Our dataset supports the ongoing use of the technique in research and clinical practice.

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ID Code: 67977
Item Type: Journal Article
Refereed: Yes
Additional Information: Abstracts of the 34th BCLA Annual Clinical Conference, Birmingham, 2010
DOI: 10.1016/j.clae.2010.07.002
ISSN: 1476-5411
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Optometry & Vision Science
Deposited On: 03 Mar 2014 04:13
Last Modified: 10 Mar 2014 22:06

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