In vitro assessment of migratory behavior of two cell populations in a simple multichannel microdevice

Kabiri, Mahboubeh, Lott, William B., Kabiri, Ehsan, Russell, Pamela J., & Doran, Michael R. (2013) In vitro assessment of migratory behavior of two cell populations in a simple multichannel microdevice. Processes, 1(3), pp. 349-359.

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Abstract

Recent literature suggests that mesenchymal stem/stromal cells (MSC) could be used as Trojan Horses to deliver “death-signals” to cancer cells. Herein, we describe the development of a novel multichannel cell migration device, and use it to investigate the relative migration rates of bone marrow-derived MSC and breast cancer cells (MCF-7) towards each other. Confluent monolayers of MSC and MCF-7 were established in adjacent chambers separated by an array of 14 microchannels. Initially, culture chambers were isolated by air bubbles (air-valves) contained within each microchannel, and then bubbles were displaced to initiate the assay. The MCF-7 cells migrated preferentially towards MSC, whilst the MSC did not migrate preferentially towards the MCF-7 cells. Our results corroborate previous literature that suggests MSC migration towards cancer cells in vivo is in response to the associated inflammation rather than directly to signals secreted by the cancer cells themselves.

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ID Code: 68228
Item Type: Journal Article
Refereed: Yes
Keywords: cancer cells; MSC migration; multichannel microdevice; paracrine signaling
DOI: 10.3390/pr1030349
ISSN: 2227-9717
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Current > Schools > School of Biomedical Sciences
Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 by the authors; licensee MDPI, Basel, Switzerland
Copyright Statement: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Deposited On: 10 Mar 2014 23:04
Last Modified: 14 Mar 2014 23:34

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