Functional hepatocyte cation compartmentation demonstrated with 133Cs NMR
Wellard, R. Mark & Adam, W.R. (2002) Functional hepatocyte cation compartmentation demonstrated with 133Cs NMR. Magnetic Resonance in Medicine, 48(5), pp. 810-818.
This study utilized the large intrinsic chemical shift range of
133Cs, a potassium congener, in an NMR study of intracellular
cation distribution. It demonstrates two distinct intracellular
environments in isolated perfused hepatocytes from cesiumfed
rats, evident as compartments with different 133Cs chemical
shifts and containing different proportions of total detected
cesium. The chemical shifts of the two intracellular compartments
were 2.44 0.07 and 1.21 0.18 ppm, relative to the
cesium signal from the perfusate. The observation of two distinct
intracellular cesium signals suggests slow exchange on an
NMR chemical shift time-scale (k exchange > 0.02 s). The area
of the high-frequency component represented 62 10% (N
12) of the total intracellular cesium signal. Manipulation of the intracellular environment using anoxia with aglycemia or digitonin produced changes in the distribution between the two intracellular compartments, showing their dynamic nature. Changes measured in association with metabolic manipulation suggest cytoplasm and mitochondria as the origin of the high and low-frequency intracellular peaks, respectively.
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