OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids
Thomas, Michael C., Mitchell, Todd W., & Blanksby, Stephen J. (2009) OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids. In Lipidomics. Humana Press (Springer), Ottowa, pp. 413-441.
Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize ozone to cleave C-C double bonds that result in chemically induced fragment ions that locate the position(s) of unsaturation
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|Item Type:||Book Chapter|
|Divisions:||Current > QUT Faculties and Divisions > Science & Engineering Faculty|
|Deposited On:||19 Mar 2014 05:25|
|Last Modified:||25 Oct 2015 16:20|
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