OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids

Thomas, Michael C., Mitchell, Todd W., & Blanksby, Stephen J. (2009) OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids. In Lipidomics. Humana Press (Springer), Ottowa, pp. 413-441.

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Abstract

Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize ozone to cleave C-C double bonds that result in chemically induced fragment ions that locate the position(s) of unsaturation

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11 citations in Scopus
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15 citations in Web of Science®

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ID Code: 68814
Item Type: Book Chapter
DOI: 10.1007/978-1-60761-322-0_21
ISBN: 978-1-60761-322-0
ISSN: 1940-6029
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Springer
Deposited On: 19 Mar 2014 05:25
Last Modified: 25 Oct 2015 16:20

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