Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry
Brown, S. H. J., Huxtable, L. H., Willcox, M. D. P., Blanksby, S. J., & Mitchell, T. W. (2013) Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry. Analyst, 138(5), pp. 1316-1320.
The deposition of biological material (biofouling) onto polymeric contact lenses is thought to be a major contributor to lens discomfort and hence discontinuation of wear. We describe a method to characterize lipid deposits directly from worn contact lenses utilizing liquid extraction surface analysis coupled to tandem mass spectrometry (LESA-MS/MS). This technique effected facile and reproducible extraction of lipids from the contact lens surfaces and identified lipid molecular species representing all major classes present in human tear film. Our data show that LESA-MS/MS is a rapid and comprehensive technique for the characterization of lipid-related biofouling on polymer surfaces.
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|Item Type:||Journal Article|
|Additional Information:||Also associated with ARC grant ARC/FT110100249|
|Keywords:||meibomian gland secretions, tear film lipids, in-vitro, hydrogel, deposition, esters, vivo, ARC, ARC FT110100249|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)|
|Divisions:||Current > QUT Faculties and Divisions > Science & Engineering Faculty|
|Copyright Owner:||Royal Society of Chemistry|
|Deposited On:||19 Mar 2014 23:42|
|Last Modified:||19 Apr 2014 11:33|
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