Ultraviolet action spectroscopy of iodine labeled peptides and proteins in the gas phase
Kirk, B. B., Trevitt, A. J., Blanksby, S. J., Tao, Y., Moore, B. N., & Julian, R. R. (2013) Ultraviolet action spectroscopy of iodine labeled peptides and proteins in the gas phase. Journal of Physical Chemistry A, 117(6), pp. 1228-1232.
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Structural investigations of large biomolecules in the gas phase are challenging. Herein, it is reported that action spectroscopy taking advantage of facile carbon-iodine bond dissociation can be used to examine the structures of large molecules, including whole proteins. Iodotyrosine serves as the active chromophore, which yields distinctive spectra depending on the solvation of the side chain by the remainder of the molecule. Isolation of the chromophore yields a double featured peak at ∼290 nm, which becomes a single peak with increasing solvation. Deprotonation of the side chain also leads to reduced apparent intensity and broadening of the action spectrum. The method can be successfully applied to both negatively and positively charged ions in various charge states, although electron detachment becomes a competitive channel for multiply charged anions. In all other cases, loss of iodine is by far the dominant channel which leads to high sensitivity and simple data analysis. The action spectra for iodotyrosine, the iodinated peptides KGYDAKA, DAYLDAG, and the small protein ubiquitin are reported in various charge states. © 2012 American Chemical Society.
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|Item Type:||Journal Article|
|Additional Information:||PubMed ID: 22906129|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)|
|Divisions:||Current > QUT Faculties and Divisions > Science & Engineering Faculty|
|Deposited On:||20 Mar 2014 03:21|
|Last Modified:||09 Dec 2014 06:22|
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