Temporal and functional changes in glycosaminoglycan expression during osteogenesis
Nurcombe, V., Goh, F. J., Haupt, Larisa M., Murali, S., & Cool, S. M. (2007) Temporal and functional changes in glycosaminoglycan expression during osteogenesis. Journal of Molecular Histology, 38(5), pp. 469-481.
Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity in turn drive phenotypic development during osteogenesis. Cells grown for 5 days under proliferating conditions were compared to cells grown for 20 days under mineralizing conditions with respect to their phenotype, the forms of HS core protein produced, and their HS sulfotransferase biosynthetic enzyme levels. RQ-PCR data was supported by the results from the purification of day 5 and day 20 HS forms by anionic exchange chromatography. The data show that cells in active growth phases produce more complex forms of sugar than cells that have become relatively quiescent during active mineralization, and that these in turn can differentially influence rates of cell growth when added exogenously back to preosteoblasts.
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|Item Type:||Journal Article|
|Additional Information:||Nurcombe, Victor
Goh, Fuqi Jack
Haupt, Larisa M
Cool, Simon M
Research Support, Non-U.S. Gov't
J Mol Histol. 2007 Oct;38(5):469-81. Epub 2007 Aug 3.
|Keywords:||3T3 Cells, Animals, Calcification, Physiologic/genetics/physiology, Cell Differentiation/drug effects/genetics/physiology, Cell Proliferation/drug effects, Extracellular Matrix/drug effects/metabolism, Fluorescent Antibody Technique, Glucuronidase/genetics/metabolism, Glycosaminoglycans/*metabolism, Heparan Sulfate Proteoglycans/genetics/metabolism, Heparitin Sulfate/isolation & purification/metabolism/pharmacology, Immunoblotting, Mice, Osteogenesis/genetics/*physiology, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, Sulfotransferases/genetics/*metabolism|
|ISSN:||1567-2387 (Online) 1567-2379 (Print)|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Development Proliferation and Death (060103)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2007 Springer Science+Business Media B.V.|
|Copyright Statement:||Author's Pre-print: author can archive pre-print (ie pre-refereeing)
Author's Post-print: author can archive post-print (ie final draft post-refereeing)
Publisher's Version/PDF: author cannot archive publisher's version/PDF
|Deposited On:||01 Apr 2014 23:03|
|Last Modified:||16 Jun 2014 03:09|
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