Strain variation amongst clinical and potable water isolates of M.kansasii using automated repetitive unit PCR
Thomson, Rachel, Tolson, Carla, Huygens, Flavia, & Hargreaves, Megan (2014) Strain variation amongst clinical and potable water isolates of M.kansasii using automated repetitive unit PCR. International Journal of Medical Microbiology, 304, pp. 484-489.
Mycobacterium kansasii is a pulmonary pathogen that has been grown readily from municipal water, but rarely isolated from natural waters. A deﬁnitive link between water exposure and disease has not been demonstrated and the environmental niche for this organism is poorly understood. Strain typing of clinical isolates has revealed seven subtypes with Type 1 being highly clonal and responsible for most infections worldwide. The prevalence of other subtypes varies geographically. In this study 49 water isolates are compared with 72 patient isolates from the same geographical area (Brisbane, Australia), using automated repetitive unit PCR (Diversilab) and ITS RFLP. The clonality of the dominant clinical strain type is again demonstrated but with rep-PCR, strain variation within this group is evident comparable with other reported methods. There is signiﬁcant heterogeneity of water isolates and very few are similar or related to the clinical isolates. This suggests that if water or aerosol transmission is the mode of infection, then point source contamination likely occurs from an alternative environmental source.
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|Item Type:||Journal Article|
|Keywords:||Mycobacterium kansasii, Nontuberculous mycobacterium, Molecular epidemiology, Genotyping, Microbial transmission|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800) > Medical Bacteriology (110801)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800) > Medical Infection Agents (incl. Prions) (110802)
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2014 Elsevier GmbH. All rights reserved.|
|Deposited On:||04 Apr 2014 01:02|
|Last Modified:||02 Oct 2014 23:42|
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