Proteomic and other analyses to determine the functional consequences of deregulated kallikrein-related peptidase (KLK) expression in prostate and ovarian cancer

Fuhrman-Luck, Ruth Anna, Silva, Munasinghage Lakmali, Dong, Ying, Irving-Rodgers, Helen, Stoll, Thomas, Hastie, Marcus Lachlan, Loessner, Daniela, Gorman, Jeffrey John, & Clements, Judith (2014) Proteomic and other analyses to determine the functional consequences of deregulated kallikrein-related peptidase (KLK) expression in prostate and ovarian cancer. Proteomics - Clinical Applications, 8(5-6), pp. 403-415.

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Abstract

Rapidly developing proteomic tools are improving detection of deregulated kallikrein-related peptidase (KLK) expression, at the protein level, in prostate and ovarian cancer, as well as facilitating the determination of functional consequences downstream. Mass spectrometry (MS)-driven proteomics uniquely allows for the detection, identification and quantification of thousands of proteins in a complex protein pool, and this has served to identify certain KLKs as biomarkers for these diseases. In this review we describe applications of this technology in KLK biomarker discovery, and elucidate MS-based techniques which have been used for unbiased, global screening of KLK substrates within complex protein pools. Although MS-based KLK degradomic studies are limited to date, they helped to discover an array of novel KLK substrates. Substrates identified by MS-based degradomics are reported with improved confidence over those determined by incubating a purified or recombinant substrate and protease of interest, in vitro. We propose that these novel proteomic approaches represent the way forward for KLK research, in order to correlate proteolysis of biological substrates with tissue-related consequences, toward clinical targeting of KLK expression and function for cancer diagnosis, prognosis and therapies.

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ID Code: 69860
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Cancer, degradomics, kallikrein, protease, proteomics
DOI: 10.1002/prca.201300098
ISSN: 1862-8346
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Funding:
  • CANCER COUNCIL QLD/1034134
Copyright Owner: Copyright 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Deposited On: 07 Apr 2014 00:34
Last Modified: 13 May 2016 08:51

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