Tandem mass spectrometry of deprotonated iodothyronines
Couldwell, A. M., Thomas, M. C., Mitchell, T. W., Hulbert, A. J., & Blanksby, Stephen J. (2005) Tandem mass spectrometry of deprotonated iodothyronines. Rapid Communications in Mass Spectrometry, 19(16), pp. 2295-2304.
In order to assist with the development of more selective and sensitive methods for thyroid hormone analysis the [M-H](-) anions of the iodothyronines T4, T3, rT3, (3,5)-T2 and the non-iodinated thyronine (TO) have been generated by negative ion electrospray mass spectrometry. Tandem mass spectra of these ions were recorded on a triple-quadrupole mass spectrometer and show a strong analogy with the fragmentation pathways of the parent compound, tyrosine. All iodothyronines also show significant abundances of the iodide anion in their tandem mass spectra, which represents an attractive target for multiple reaction monitoring (MRM) analysis, given that iodothyronines are the only iodine bearing endogenous molecules. Characteristic fragments are observed at m/z 359.7 and 604.5 for rT3 but are absent in the spectrum of T3, thus differentiating the two positional isomers. The striking difference in the fragmentation patterns of these regioisomeric species is attributed to the increased acidity of the phenol moiety in rT3 compared with T3. Copyright (C) 2005 John Wiley & Sons, Ltd.
Impact and interest:
Citation counts are sourced monthly from and citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|Item Type:||Journal Article|
|Keywords:||collision-induced dissociations, alpha-amino-acids, thyroid-hormones, electrospray-ionization, anionic migration, total thyroxine, gas-phase, serum, ion, fragmentations|
|ISSN:||1097-0231 (online) 0951-4198 (print)|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2005 John Wiley & Sons, Ltd.|
|Deposited On:||13 May 2014 01:34|
|Last Modified:||30 Jan 2016 21:17|
Repository Staff Only: item control page