High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris

Abad, Sandra, Nahalka, Jozef, Winkler, Margit, Bergler, Gabriele, Speight, Robert, Glieder, Anton, & Nidetzky, Bernd (2010) High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris. Biotechnology Letters, 33(3), pp. 557-563.

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Abstract

By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pastoris cells permeabilized by freeze-drying and incubation in 2-propanol (10% v/v) produce a highly active (1.6 kUnits/g dry matter) and stable oxidase preparation. Critical bottlenecks in the development of an RgDAO catalyst for industrial applications have been eliminated.

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6 citations in Web of Science®

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ID Code: 70405
Item Type: Journal Article
Refereed: Yes
DOI: 10.1007/s10529-010-0456-9
ISSN: 1573-6776
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Springer
Deposited On: 22 Apr 2014 05:20
Last Modified: 22 May 2014 07:06

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