Effect of culture conditions and calcium phosphate coating on ectopic bone formation

Vaquette, Cedryck, Ivanovski, Saso, Hamlet, Stephen M., & Hutmacher, Dietmar W. (2013) Effect of culture conditions and calcium phosphate coating on ectopic bone formation. Biomaterials, 34(22), pp. 5538-5551.

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This study investigated the effect of a calcium phosphate (CaP) coating onto a polycaprolactone melt electrospun scaffold and in vitro culture conditions on ectopic bone formation in a subcutaneous rat model. The CaP coating resulted in an increased alkaline phosphatase activity (ALP) in ovine osteoblasts regardless of the culture conditions and this was also translated into higher levels of mineralisation. A subcutaneous implantation was performed and increasing ectopic bone formation was observed over time for the CaPcoated samples previously cultured in osteogenic media whereas the corresponding non-coated samples displayed a lag phase before bone formation occurred from 4 to 8 weeks post-implantation. Histology and immunohistochemistry revealed bone fill through the scaffolds 8 weeks post-implantation for coated and non-coated specimens and that ALP, osteocalcin and collagen 1 were present at the ossification front and in the bone tissues. Vascularisation in the vicinity of the bone tissues was also observed indicating that the newly formed bone was not deprived of oxygen and nutrients.We found that in vitro osteogenic induction was essential for achieving bone formation and CaP coating accelerated the osteogenic process. We conclude that high cell density and preservation of the collagenous and mineralised extracellular matrix secreted in vitro are factors of importance for ectopic bone formation.

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ID Code: 70503
Item Type: Journal Article
Refereed: Yes
Keywords: Melt electrospinning, Calcium phosphate, Ectopic bone formation, Surface modification
DOI: 10.1016/j.biomaterials.2013.03.088
ISSN: 01429612
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Elsevier Ltd.
Copyright Statement: This is the author’s version of a work that was accepted for publication in Biomaterials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials, [VOL 34, ISSUE 22, (2013)] DOI: 10.1016/j.biomaterials.2013.03.088
Deposited On: 28 Apr 2014 00:48
Last Modified: 28 Apr 2014 00:48

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