Controlling microencapsulation and release of micronized proteins using poly(ethylene glycol) and electrospraying

Bock, Nathalie, Dargaville, Tim R., & Woodruff, Maria A. (2014) Controlling microencapsulation and release of micronized proteins using poly(ethylene glycol) and electrospraying. European Journal of Pharmaceutics and Biopharmaceutics, 87(2), pp. 366-377.

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The fabrication of tailored microparticles for delivery of therapeutics is a challenge relying upon a complex interplay between processing parameters and materials properties. The emerging use of electrospraying allows better tailoring of particle morphologies and sizes than current techniques, critical to reproducible release profiles. While dry encapsulation of proteins is essential for the release of active therapeutics from microparticles, it is currently uncharacterized in electrospraying. To this end, poly(ethylene glycol) (PEG) was assessed as a micronizing and solubilizing agent for dry protein encapsulation and release from electrosprayed particles made from polycaprolactone (PCL). The physical effect of PEG in protein-loaded poly(lactic-co-glycolic acid) (PLGA) particles was also studied, for comparison. The addition of 5–15 wt% PEG 6 kDa or 35 kDa resulted in reduced PCL particle sizes and broadened distributions, which could be improved by tailoring the electrospraying processing parameters, namely by reducing polymer concentration and increasing flow rate. Upon micronization, protein particle size was reduced to the micrometer domain, resulting in homogenous encapsulation in electrosprayed PCL microparticles. Microparticle size distributions were shown to be the most determinant factor for protein release by diffusion and allowed specific control of release patterns.

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ID Code: 70760
Item Type: Journal Article
Refereed: Yes
Keywords: Electrospraying; Drug delivery; Encapsulation; In vitro release; Microparticles; Micronization; Polycaprolactone; Poly(ethylene glycol); Protein
DOI: 10.1016/j.ejpb.2014.03.008
ISSN: 0939-6411
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomedical Engineering not elsewhere classified (090399)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > PHARMACOLOGY AND PHARMACEUTICAL SCIENCES (111500) > Pharmacology and Pharmaceutical Sciences not elsewhere classified (111599)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2014 Elsevier B.V.
Deposited On: 30 Apr 2014 23:58
Last Modified: 17 Dec 2014 01:55

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