High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates

D’Sa, Dexter J., de Juan Pardo, Elena M., de las Rivas Astiz, Rosalia, Sen, Shamik, & Kumar, Sanjay (2009) High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates. Applied Physics Letters, 95(6), 063701.

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Much interest surrounds the effect of extracellular matrix (ECM) elasticity on cell behavior. Here we present a rapid method for measuring the elasticity of synthetic ECM substrates based on indentation of the substrate with a ferromagnetic sphere and optical tracking of the resulting deformation. We find that this method yields order-of-magnitude agreement with atomic force microscopy elasticity measurements, but that the degree of this agreement depends strongly on sphere density and gel elasticity. In its regime of greatest accuracy, we envision that this method may be used for high-throughput characterization of ECM substrates in cell biological studies.

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4 citations in Scopus
2 citations in Web of Science®
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ID Code: 70780
Item Type: Journal Article
Refereed: Yes
DOI: 10.1063/1.3197013
ISSN: 0003-6951
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2009 American Institute of Physics.
Deposited On: 30 Apr 2014 23:07
Last Modified: 30 May 2014 04:39

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