High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates
D’Sa, Dexter J., de Juan Pardo, Elena M., de las Rivas Astiz, Rosalia, Sen, Shamik, & Kumar, Sanjay (2009) High-throughput indentational elasticity measurements of hydrogel extracellular matrix substrates. Applied Physics Letters, 95(6), 063701.
Much interest surrounds the effect of extracellular matrix (ECM) elasticity on cell behavior. Here we present a rapid method for measuring the elasticity of synthetic ECM substrates based on indentation of the substrate with a ferromagnetic sphere and optical tracking of the resulting deformation. We find that this method yields order-of-magnitude agreement with atomic force microscopy elasticity measurements, but that the degree of this agreement depends strongly on sphere density and gel elasticity. In its regime of greatest accuracy, we envision that this method may be used for high-throughput characterization of ECM substrates in cell biological studies.
Impact and interest:
Citation counts are sourced monthly from and citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2009 American Institute of Physics.|
|Deposited On:||30 Apr 2014 23:07|
|Last Modified:||30 May 2014 04:39|
Repository Staff Only: item control page