Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair

Liu, Fangjun, Porter, Ryan M., Wells, James, Glatt, Vaida, Pilapil, Carmencita, & Evans, Christopher H. (2011) Evaluation of BMP-2 gene-activated muscle grafts for cranial defect repair. Journal of Orthopaedic Research, 30(7), pp. 1095-1102.

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Large, osseous, segmental defects heal poorly. Muscle has a propensity to form bone when exposed to an osteogenic stimulus such as that provided by transfer and expression of cDNA encoding bone morphogenetic protein-2 (BMP-2). The present study evaluated the ability of genetically modified, autologous muscle to heal large cranial defects in rats. Autologous grafts (8 mm � 2 mm) were punched from the biceps femoris muscle and transduced intraoperatively with recombinant adenovirus vector containing human BMP-2 or green fluorescent protein cDNA. While the muscle biopsies were incubating with the vector, a central parietal 8 mm defect was surgically created in the calvarium of the same animal. The gene-activated muscle graft was then implanted into the cranial defect. After 8 weeks, crania were examined radiographically, histologically, and by micro-computed tomography and dual energy X-ray absorptiometry. Although none of the defects were completely healed in this time, muscle grafts expressing BMP-2 deposited more than twice as much new bone as controls. Histology confirmed the anatomical integrity of the newly formed bone, which was comparable in thickness and mineral density to the original cranial bone. This study confirms the in vivo osteogenic properties of genetically modified muscle and suggests novel strategies for healing bone. � 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1095–1102, 2012

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16 citations in Scopus
18 citations in Web of Science®
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ID Code: 70856
Item Type: Journal Article
Refereed: Yes
Keywords: gene therapy, bone healing, muscle, BMP-2, osteogenesis
DOI: 10.1002/jor.22038
ISSN: 0736-0266
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: � 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Deposited On: 01 May 2014 03:04
Last Modified: 21 Jun 2017 03:01

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