Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics

Wulsten, Dag, Glatt, Vaida, Ellinghaus, Agnes, Schmidt-Bleek, Katharina, Petersen, Ansgar, Schell, Hanna, Lienau, Jasmin, Sebald, Walter, Plöger, Frank, Seemann, Petra, & Duda, Georg N. (2011) Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics. European Cells and Materials, 21, pp. 177-192.

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Abstract

This study reports that treatment of osseous defects with different growth factors initiates distinct rates of repair. We developed a new method for monitoring the progression of repair, based upon measuring the in vivo mechanical properties of healing bone. Two different members of the bone morphogenetic protein (BMP) family were chosen to initiate defect healing: BMP-2 to induce osteogenesis, and growth-and-differentiation factor (GDF)-5 to induce chondrogenesis. To evaluate bone healing, BMPs were implanted into stabilised 5 mm bone defects in rat femurs and compared to controls. During the first two weeks, in vivo biomechanical measurements showed similar values regardless of the treatment used. However, 2 weeks after surgery, the rhBMP-2 group had a substantial increase in stiffness, which was supported by the imaging modalities. Although the rhGDF-5 group showed comparable mechanical properties at 6 weeks as the rhBMP-2 group, the temporal development of regenerating tissues appeared different with rhGDF-5, resulting in a smaller callus and delayed tissue mineralisation. Moreover, histology showed the presence of cartilage in the rhGDF-5 group whereas the rhBMP-2 group had no cartilaginous tissue.

Therefore, this study shows that rhBMP-2 and rhGDF-5 treated defects, under the same conditions, use distinct rates of bone healing as shown by the tissue mechanical properties. Furthermore, results showed that in vivo biomechanical method is capable of detecting differences in healing rate by means of change in callus stiffness due to tissue mineralisation.

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17 citations in Web of Science®

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ID Code: 70858
Item Type: Journal Article
Refereed: Yes
Keywords: Large segmental defects, bone healing, bone morphogenetic protein, growth and differentiation factor, small animal model
ISSN: 1473-2262
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2011 [Please consult the author]
Deposited On: 01 May 2014 23:20
Last Modified: 19 May 2014 03:19

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