Direct and quantitative detection of bacteriophage by “hearing” surface detachment using a quartz crystal microbalance

Dultsev, F. N., Speight, R. E., Fiorini, M. T., Blackburn, J. M., Abell, C., Ostanin, V. P., & Klenerman, D. (2001) Direct and quantitative detection of bacteriophage by “hearing” surface detachment using a quartz crystal microbalance. Analytical Chemistry, 73(16), pp. 3935-3939.

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We show that it is possible to detect specifically adsorbed bacteriophage directly by breaking the interactions between proteins displayed on the phage coat and ligands immobilized on the surface of a quartz crystal microbalance (QCM). This is achieved through increasing the amplitude of oscillation of the QCM surface and sensitively detecting the acoustic emission produced when the bacteriophage detaches from the surface. There is no interference from nonspecifically adsorbed phage. The detection is quantitative over at least 5 orders of magnitude and is sensitive enough to detect as few as 20 phage. The method has potential as a sensitive and low-cost method for virus detection.

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45 citations in Web of Science®

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ID Code: 70987
Item Type: Journal Article
Refereed: Yes
Keywords: biochemical methods
DOI: 10.1021/ac0100897
ISSN: 1520-6882
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 04 May 2014 23:01
Last Modified: 04 May 2014 23:01

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