Recombinant cellulase accumulation in the leaves of mature, vegetatively propagated transgenic sugarcane

Harrison, Mark D., Geijskes, R. Jason, Lloyd, Robyn, Miles, Stacy, Palupe, Anthony, Sainz, Manuel B., & Dale, James L. (2014) Recombinant cellulase accumulation in the leaves of mature, vegetatively propagated transgenic sugarcane. Molecular Biotechnology, 56(9), pp. 795-802.

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Abstract

The cost of enzymes that hydrolyse lignocellulosic substrates to fermentable sugars needs to be reduced to make cellulosic ethanol a cost-competitive liquid transport fuel. Sugarcane is a perennial crop and the successful integration of cellulase transgenes into the sugarcane production system requires that transgene expression is stable in the ratoon. Herein, we compared the accumulation of recombinant fungal cellobiohydrolase I (CBH I), fungal cellobiohydrolase II (CBH II), and bacterial endoglucanase (EG) in the leaves of mature, initial transgenic sugarcane plants and their mature ratoon. Mature ratoon events containing equivalent or elevated levels of active CBH I, CBH II, and EG in the leaves were identified. Further, we have demonstrated that recombinant fungal CBH I and CBH II can resist proteolysis during sugarcane leaf senescence, while bacterial EG cannot. These results demonstrate the stability of cellulase enzyme transgene expression in transgenic sugarcane and the utility of sugarcane as a biofactory crop for production of cellulases.

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2 citations in Scopus
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3 citations in Web of Science®

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ID Code: 70998
Item Type: Journal Article
Refereed: Yes
Keywords: Sugarcane, Transgenic, Cellulase, Cellulosic ethanol, Biofuel, Expression
DOI: 10.1007/s12033-014-9758-9
ISSN: 1073-6085
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Enzymes (060107)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > CROP AND PASTURE PRODUCTION (070300) > Crop and Pasture Biomass and Bioproducts (070304)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Institutes > Institute for Future Environments
Copyright Owner: Copyright 2014 Springer
Deposited On: 05 May 2014 00:53
Last Modified: 20 Dec 2014 02:09

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